Toxicological Chemistry

Study Course Description

Course Description Statuss:Approved

Course Description Version:9.00

Study Course Accepted:21.03.2024 08:58:00

Study Course Information
Course Code:		FKK_012		LQF level:		Level 7
Credit Points:		5.00		ECTS:		7.50
Branch of Science:		Basic Sciences of Medicine, including Pharmacy		Target Audience:		Pharmacy
Study Course Supervisor
Course Supervisor:		Katrīna Sudmale
Study Course Implementer
Structural Unit:		Department of Pharmaceutical Chemistry
The Head of Structural Unit:
Contacts:		Riga, 16 Dzirciema Street, Block A, 5th floor, Room no 502, farmkkrsu[pnkts]lv, +371 67061544
Study Course Planning
Full-Time - Semester No.1
Lectures (count)		6	Lecture Length (academic hours)		2	Total Contact Hours of Lectures		12
Classes (count)		5	Class Length (academic hours)		4	Total Contact Hours of Classes		20
Total Contact Hours								32
Full-Time - Semester No.2
Lectures (count)		0	Lecture Length (academic hours)		0	Total Contact Hours of Lectures		0
Classes (count)		12	Class Length (academic hours)		4	Total Contact Hours of Classes		48
Total Contact Hours								48
Part-Time - Semester No.1
Lectures (count)		8	Lecture Length (academic hours)		4	Total Contact Hours of Lectures		32
Classes (count)		8	Class Length (academic hours)		4	Total Contact Hours of Classes		32
Total Contact Hours								64
Study course description
Preliminary Knowledge:
Inorganic, organic and analytical chemistry, human anatomy, physiology and pathology.
Objective:
• to promote the acquisition of knowledge on the main methods of analysis for the determination of toxic substances in biological objects • to promote awareness of the possibilities of chemical and physico-chemical analysis methods in toxicological analysis • to master the types of tasks of the issues included in the course and the methodology of their solution
Topic Layout (Full-Time)
No.	Topic			Type of Implementation		Number	Venue
1	Toxicology and analytical toxicology. Dose-response relationship.			Lectures		1.00	auditorium
2	Analytical toxicology of volatile toxicants.			Lectures		1.00	auditorium
3	Physio-chemical properties and methods of isolation of non-volatile organic toxicants.			Lectures		1.00	auditorium
4	Chromatographic and spectral methods in analysis of drugs and other organic toxicants.			Lectures		1.00	auditorium
5	Analytical toxicology of toxic cations, anions and pesticides.			Lectures		1.00	auditorium
6	Essentials of toxicokinetics and toxicodynamics for interpretation of analytical results.			Lectures		1.00	auditorium
7	Isolation and identification of volatile toxicants. Analysis of volatile toxicants.			Classes		0.50	laboratory
8	Colloquium. General principles of analytical toxicology. Volatile toxicants.			Classes		1.00	laboratory
9	Isolation of non volatile organic toxicants. Analysis of drugs in biological samples.			Classes		0.50	laboratory
10	Colloquium. Isolation and analysis of non-volatile organic toxicants.			Classes		1.00	laboratory
11	Identification of non-volatile organic toxic substances.			Classes		0.50	laboratory
12	Colloquium. Chemical-toxicological analysis of poisonous cations, anions and pesticides.			Classes		1.00	laboratory
13	Mineralization of biological material.			Classes		0.50	laboratory
14	Isolation and analysis of volatile toxic compounds. Control analysis (volatile poisons).			Classes		0.50	laboratory
15	Isolation of non-volatile organic toxicants.			Classes		0.50	laboratory
16	Identification of non-volatile organic toxicants. Control analysis (non-volatile organic poisons).			Classes		0.50	laboratory
17	Isolation and demonstration of non-volatile organic toxicants.			Classes		0.50	laboratory
18	Detection of toxic cations in minerals. Control analysis (metallic poisons).			Classes		0.50	laboratory
19	Detection of toxic anions. Control analysis.			Classes		0.50	laboratory
20	Control analysis: Isolation and demonstration of volatile compounds. Full forensic chemical analysis.			Classes		1.00	laboratory
21	Control analysis: Isolation and demonstration of drug substances.			Classes		0.50	laboratory
22	Control analysis: isolation and detection of toxic cations.			Classes		0.50	laboratory
23	Colloquium: Isolation and identification of toxicants.			Classes		1.00	laboratory
24	Detection of toxic compounds in urine by colour reactions. Control analysis: Express methods in the diagnosis of poisoning.			Classes		1.00	laboratory
25	Detection of drug substances in urine by solid phase extraction and thin layer chromatography screening. Control test: Identification of the toxic compound.			Classes		1.00	laboratory
26	Detection of therapeutic substances in urine by extraction and electron spectroscopy. Control analysis: Identification of toxicants in urine by their UV spectra.			Classes		1.00	laboratory
27	Seminar on toxicokinetics. Test on toxicokinetics.			Classes		0.50	laboratory
28	Seminar on toxicant metabolism. Test on metabolism.			Classes		0.50	laboratory
29	Colloquium: Toxicokinetics and toxicodynamics.			Classes		1.00	laboratory
30	Use of infrared spectroscopy for identification of toxicants. Control analysis: Identification of drug substances by IS spectra.			Classes		1.00	laboratory
Topic Layout (Part-Time)
No.	Topic			Type of Implementation		Number	Venue
1	Toxicology and analytical toxicology. Dose-response relationship.			Lectures		1.00	auditorium
2	Analytical toxicology of volatile toxicants.			Lectures		1.50	auditorium
3	Physio-chemical properties and methods of isolation of non-volatile organic toxicants.			Lectures		1.50	auditorium
4	Chromatographic and spectral methods in analysis of drugs and other organic toxicants.			Lectures		1.50	auditorium
5	Analytical toxicology of toxic cations, anions and pesticides.			Lectures		1.00	auditorium
6	Essentials of toxicokinetics and toxicodynamics for interpretation of analytical results.			Lectures		1.50	auditorium
7	Isolation and identification of volatile toxicants. Analysis of volatile toxicants.			Classes		0.50	laboratory
8	Colloquium. General principles of analytical toxicology. Volatile toxicants.			Classes		0.50	laboratory
9	Isolation of non volatile organic toxicants. Analysis of drugs in biological samples.			Classes		0.50	laboratory
10	Colloquium. Isolation and analysis of non-volatile organic toxicants.			Classes		0.50	laboratory
11	Identification of non-volatile organic toxic substances.			Classes		0.50	laboratory
12	Colloquium. Chemical-toxicological analysis of poisonous cations, anions and pesticides.			Classes		0.50	laboratory
13	Mineralization of biological material.			Classes		1.00	laboratory
14	Isolation and analysis of volatile toxic compounds. Control analysis (volatile poisons).			Classes		0.50	laboratory
15	Isolation of non-volatile organic toxicants.			Classes		0.50	laboratory
19	Detection of toxic anions. Control analysis.			Classes		0.50	laboratory
20	Control analysis: Isolation and demonstration of volatile compounds. Full forensic chemical analysis.			Classes		0.50	laboratory
24	Detection of toxic compounds in urine by colour reactions. Control analysis: Express methods in the diagnosis of poisoning.			Classes		0.50	laboratory
26	Detection of therapeutic substances in urine by extraction and electron spectroscopy. Control analysis: Identification of toxicants in urine by their UV spectra.			Classes		0.50	laboratory
28	Seminar on toxicant metabolism. Test on metabolism.			Classes		0.50	laboratory
29	Colloquium: Toxicokinetics and toxicodynamics.			Classes		0.50	laboratory
Assessment
Unaided Work:
Individual work in the laboratory, performing experiments, solving situation tasks. It is recommended to fill in the study course survey, evaluating the existing course content and creating proposals for updating the course.
Assessment Criteria:
1. Colloquia and seminars on toxic effects, their isolation, analysis, data interpretation. (30%) 2. Solving situation tasks. (10%) 3. Practical isolation of toxicants from biological objects, their analysis and interpretation of results. (10%) 4. Exam: knowledge of the mechanisms of action of toxicants, their isolation from the section material, qualitative and quantitative analysis, the use of regularities of toxicokinetics and toxicodynamics in interpreting the results of analyses are tested. (50%)
Final Examination (Full-Time):		Exam (Written)
Final Examination (Part-Time):		Exam (Written)
Learning Outcomes
Knowledge:		After completing the study course, students will be able to: • evaluate the objects to be used in the toxicological analysis; • present the theoretical basis of the methods used in the analysis; • understand and interpret analytical methodologies available in the literature.
Skills:		As a result of mastering the study course students: • will be able to isolate different types (volatile, organic, non-volatile, metal-containing) toxicants from biological objects; • will be able to use chromatographic, spectroscopic methods and chromogenic reactions in an experiment to detect the most well-known toxicants; • will be able to apply toxicokinetics and toxicodynamics to the interpretation of the results of legality analyses.
Competencies:		As a result of the study course, students will be able to isolate the most well-known toxicants from biological objects, analyse and interpret the results.
Bibliography
No.	Reference
Required Reading
1	Casarett and Doull's toxicology: the basic science of poisons / editor, Curtis D. Klaassen. 9th ed. New York: McGraw-Hill Education, 2019. xiii, 1454 lpp.
2	V.Liguts. Toksikoloģijas rokasgrāmata. Rīga, 2001. (akceptējams izdevums)
3	S.Jickels, A.Negrusz (eds). Clarke’s Analytical Forensic Toxicology. 2nd ed. Pharmaceutical Press, London, Chicago, 2013.
4	R.J.Flanagan, et al. Fundamentals of Analytical Toxicology. Willey, 2020
5	Ārvalstu studentiem/For international students
6	Casarett and Doull's toxicology: the basic science of poisons / editor, Curtis D. Klaassen. 9th ed. New York: McGraw-Hill Education, 2019. xiii, 1454 lpp.
7	S.Jickels, A.Negrusz (eds). Clarke’s Analytical Forensic Toxicology. 2nd ed. Pharmaceutical Press, London, Chicago, 2013.
Additional Reading
1	J.A.Timbrell. Introduction to Toxicology. London, 2009
2	A. C. Moffat, et al. (eds). Clarke’s Analysis of Drugs and Poisons. London, Chicago, 2011.