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Human Molecular Biology

Study Course Description

Course Description Statuss:Approved
Course Description Version:5.00
Study Course Accepted:28.07.2020
Study Course Information
Course Code:BUMK_045LQF level:Level 6
Credit Points:4.00ECTS:6.00
Branch of Science:Biology; Molecular BiologyTarget Audience:Dentistry
Study Course Supervisor
Course Supervisor:Rudīte Koka
Study Course Implementer
Structural Unit:Department of Biology and Microbiology
The Head of Structural Unit:Juta Kroiča
Contacts:Rīga, Dzirciema iela 16, bmk@rsu.lv, +371 67061584
Study Course Planning
Full-Time - 1. Semester No.
Lectures (count)7Lecture Length (academic hours)2Total Contact Hours of Lectures14
Classes (count)9Class Length (academic hours)2Total Contact Hours of Classes18
Total Contact Hours32
Lectures (count)6Lecture Length (academic hours)2Total Contact Hours of Lectures12
Classes (count)10Class Length (academic hours)2Total Contact Hours of Classes20
Total Contact Hours32
Study course description
Preliminary Knowledge:
Knowledge of biology acquired within the framework of general secondary education.
Objective:
To make understanding about molecular processes in cell, linkage between cell function and provision of homeostasis in human body, as well as understanding about principles of human genome structure and role of disturbances in genome in etiology of genetic pathologies, to develop skills of students in work with methods of molecular biology, to help to understand the use of theoretical knowledge in medical practice, to help to understand role of science in medical development and use of scientific opinion in practice of a medical doctor.
Topic Layout (Full-Time)
No.TopicType of ImplementationNumberVenue
1The subject of human biology. The cells plasma membrane. Cells types. Transport across membranes.Lectures1.00auditorium
2The Nucleus. Chromatin. Chromosomes. Human karyotype.Lectures1.00auditorium
3Molecular basis of heredity. The structure of DNA and RNA. DNA replication. Transcription.Lectures1.00auditorium
4Protein biosynthesis. Translation. Genetic code.Lectures1.00auditorium
5Regulation of gene expression in eukaryotic cell. DNA repair, mechanisms and diseases.Lectures1.00auditorium
6Cell cycle; regulation of cell cycle. Cell division. Mitosis. Meiosis.Lectures1.00auditorium
7Gametogenesis. Abnormal cell division – the reason for chromosomal abnormalities.Lectures1.00auditorium
8The subject of genetics. Basics of classical genetics. Allelic gene ineraction.Lectures1.00auditorium
9Nonallelic gene ineraction. Gene linkage. Inheritance of traits in case of gene linkage.Lectures1.00auditorium
10Types of inheritance. Transmission of autosomal and sex-linked characteristics. Pedigree analysis.Lectures1.00auditorium
11Multifactorial type of inheritance. Multifactorial traits in humans. Case analysis.Lectures1.00auditorium
12Variations of the human genome. Chromosome and genome mutations. Independent learning Chromosomal diseases.Lectures1.00auditorium
13Population genetics.Lectures1.00auditorium
14Structure of procaryotic and eucaryotic cells (microscopic slides).Classes1.00laboratory
16Water transport in the eucaryotic cell (practical work).Classes1.00laboratory
17Analysis of Human karyotype. Analysis of metaphase plates. Politen chromosomes (microscopic slides).Classes1.00laboratory
18DNA isolation. Laboratory work.Classes1.00laboratory
19Molecular biology. DNA sequence analysis.Classes1.00laboratory
20Colloquium I (cytology).Classes1.00auditorium
21Analysis of X chromatin (temporary slides; optional), human karyotype; Barr bodies (microscopic slides).Classes1.00laboratory
22Analysis of stages in gametogenesis (microscopic slides, posters). Mature sex cells (microscopic slides).Classes1.00laboratory
23Colloquium 2.Classes1.00laboratory
24Problem solving: monoyhybrid-, diyhybrid, polyhybrid crosses.Classes1.00laboratory
25Problem solving: interaction of allelic and non-allelic genes.Classes1.00laboratory
26Problem solving: gene linkage.Classes1.00laboratory
27Pedigree analysis I. (Autosomal types of inheritance).Classes1.00laboratory
28Pedigree analysis II. (X chromosome linked and holandric types of inheritance).Classes1.00laboratory
29Colloquium (genetics nr. 1).Classes1.00laboratory
30Multifactorial type of inheritance. Multifactorial traits in humans. Case analysis.Classes1.00laboratory
31Chromosome and genome mutations. Chromosomal diseases. Problem solving.Classes1.00laboratory
32Population genetics. Problem solving.Classes1.00laboratory
33Colloquium (genetics no. 2).Classes1.00laboratory
Assessment
Unaided Work:
In the 1st semester: independent acquisition of the topics Structure of cytoplasm of the eukaryotic cell, the structure and functions of organoids. In each lesson, according to the topic, the student receives assignments for independent learning. Task solutions must be used genetic code, its properties. Preparation of presentations in 1st semester about Organelles of eukaryotic cells and their function. 2nd semester about multifactorial diseases in accordance with the criteria discussed during classes.
Assessment Criteria:
Participation in classes and compliance with methodology during laboratory works; weekly tests of theoretical knowledge, ability to explain the results obtained during practical works and to draw conclusions based on the results mentioned above. In the 1st semester the students' knowledge and skills will be evaluated during colloquia: • 1st colloquium: a control class in cytology I (a written theoretical part); • 2nd colloquium: a control class in cytology II (a written theoretical part and solution of tasks). In the 2nd semester the students' skills and knowledge will be evaluated during 2 colloquia: • 1st colloquia: about classification of types of inheritance (a written theoretical part and solution of tasks); • 2nd colloquium: a control class about chromosomal pathology, gene action in population (a written theoretical part and solution of tasks). The following skills will be evaluated: ability to present the prepared material in accordance with the theme and ability to apply theoretical knowledge in solving genetic tasks. A written examination at the end of the course. The examination consists of the themes covered during only 2nd semester. Cumulative assessment of the examination: assessment of colloquia.
Final Examination (Full-Time):Exam (Written)
Final Examination (Part-Time):
Learning Outcomes
Knowledge:• Students analyse the conformity of eukaryotic cell structure with its functions; • justify interrelation of the cell structure and the role of abnormal function in human pathology; • compare processes in mitosis and meiosis; • know the main conformities of genetics; • evaluate the role of Mendel's experiments in the development of genetics; • compare the results of monohybrid, dihybrid, polyhybrid and analytic crosses; • explain types of interaction of allelic and non-allelic genes and impact of interaction on the manifestation of features in phenotype; • analyse the interconnection between genes; • know the primary and secondary structure of nucleic acids; • explain the replication, transcription, translation of DNA and regulation of gene expression; • recognise chromosomal pathologies, analyse possible mechanisms of origin and consequences; • distinguish factors of disarrangement of gene balance in population, know the types of selection and describe the selection in human population; • explain the application of genetic cognitions in medical practice; • justify the manifestations of mutations of development genes in early stages of onthogenesis; • explain the impact of development genes on the formation of the human body; • defend the opinion while analysing particular situations regarding inheriting of human features; • apply conceptions and terms discussed during classes.
Skills:Upon successful completion of the course students will be able to: • use light microscope in order to identify specific structures of eukaryote cell, metaphase chromosomes, polytene chromosomes; explain the connection of structure and functions; • portray the phase of mitosis and meiosis graphically and explain the on-going processes; • create the schemes of oogenesis and spermogenesis, compile and analyse family medical tree; present the etiology of diseases resulting from multifactorial inheritance, possible causes, single out and prove DNA, classify types of changeability and mutation; • discuss about the creation mechanisms of genes, chromosomes, genome mutations; solve the tasks regarding genetics and molecular biology.
Competencies:Students will be able to analyse situations in medical pathology, genetics, evaluate scientific achievements and the role of genetics in dental practice.
Bibliography
No.Reference
Required Reading
1A. Krūmiņa., V. Baumanis. Eikariotu šūnu bioloģija. Rīga. RSU. 2015.
2Tsisana Shartava. DNA Research, Genetics, and Cell Biology. Nova Science Publishers, 2011. RSU. E-grāmata. datu bāze: EBSCO.
3Molecular Biology, Nanette J. Pazdernik. RSU E-book. Datu bāze: ClinicalKey&EBRARY
Additional Reading
1Benjamin A. Pierce. Genetics A Conceptual Approach. Fifth Edition. Southwestern University. 2014.
2Pollard T.D., Earnshaw W.C. Cell biology. 3rd edition. Elsevier Science (USA), 2016.-908 p.
3Evans J., Manson A.L. Cell Biology and Genetics. Mosby, Elsevier, 2008.
4Selga Tūrs. Šūnu bioloģija. LU akadēmiskais apgāds. 2007.- 342.lpp.
Other Information Sources
1Internetā atslēgvārdi- DNA &RNA, Genetics&Medicine, Genes &Expression, Genomes&Maps.
2http://estudijas. rsu.lv. --> Zobārstniecība--> 1. studiju gads-->cilvēka molekulārā bioloģija