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Biochemistry and Laboratory Diagnostics

Study Course Description

Course Description Statuss:Approved
Course Description Version:4.00
Study Course Accepted:14.03.2024 11:44:18
Study Course Information
Course Code:SL_102LQF level:Level 7
Credit Points:2.00ECTS:3.00
Branch of Science:Clinical MedicineTarget Audience:Life Science
Study Course Supervisor
Course Supervisor:Angelika Krūmiņa
Study Course Implementer
Structural Unit:Statistics Unit
The Head of Structural Unit:
Contacts:23 Kapselu street, 2nd floor, Riga, statistikaatrsu[pnkts]lv, +371 67060897
Study Course Planning
Full-Time - Semester No.1
Lectures (count)4Lecture Length (academic hours)2Total Contact Hours of Lectures8
Classes (count)8Class Length (academic hours)2Total Contact Hours of Classes16
Total Contact Hours24
Part-Time - Semester No.1
Lectures (count)4Lecture Length (academic hours)1Total Contact Hours of Lectures4
Classes (count)8Class Length (academic hours)1Total Contact Hours of Classes8
Total Contact Hours12
Study course description
Preliminary Knowledge:
Knowledge in chemistry, human physiology, biology, mathematics, physics.
Objective:
To facilitate the acquisition of theoretical knowledge about chemical processes in the human body in relation to its functions. To improve the understanding of modern laboratory diagnostic possibilities and their interpretation by providing information on the chemical composition of materials used in diagnostics.
Topic Layout (Full-Time)
No.TopicType of ImplementationNumberVenue
1Carbohydrate metabolism – digestion and absorption of carbohydrates, glycogen metabolism (glycogenolysis, glycogenogenesis), glycolysis, pentose pathway, gluconeogenesis. Functions of carbohydrates in the human body.Lectures1.00auditorium
2Lipid metabolism – digestion and absorption, lipolysis and lipogenesis, phospholipid and cholesterol metabolism, fatty acids and ketones. Functions of lipids in the human body.Lectures1.00auditorium
3Protein metabolism: digestion and absorption, biosynthesis of amino acids and complex proteins. Functions of proteins in the human body.Lectures1.00auditorium
4Possibilities of laboratory diagnostics: classification, material and methods used, their specificity and sensitivity.Lectures1.00auditorium
5Complete blood count, blood biochemistry, blood culture, cytogenetic examination – indicators, meaning thereof, interpretation of changes.Classes1.00auditorium
6Fecal examination: fecal analysis, hidden blood, culture, biochemistry – indicators, meaning thereof, interpretation of changes.Classes1.00auditorium
7Urine analysis, the so-called microscopy, urine culture – indicators, meaning thereof, interpretation of changes.Classes1.00auditorium
8Serological test: antibody formation, antibody classes. Rapid and confirmatory tests.Classes1.00auditorium
9Laboratory diagnostics of M.tuberculosis – available methods, time of implementation and accuracy thereof. Introduction to polymer chain reaction (PCR).Classes1.00auditorium
10Histological examination – definition, indications, methods to be used, interpretation of results.Classes1.00auditorium
11Punctures: therapeutic and diagnostic indications. Type of collection. Examination possibilities. Interpretation of results.Classes1.00auditorium
12Bone marrow examination – indications, abnormalities and their interpretation.Classes1.00auditorium
Topic Layout (Part-Time)
No.TopicType of ImplementationNumberVenue
1Carbohydrate metabolism – digestion and absorption of carbohydrates, glycogen metabolism (glycogenolysis, glycogenogenesis), glycolysis, pentose pathway, gluconeogenesis. Functions of carbohydrates in the human body.Lectures1.00auditorium
2Lipid metabolism – digestion and absorption, lipolysis and lipogenesis, phospholipid and cholesterol metabolism, fatty acids and ketones. Functions of lipids in the human body.Lectures1.00auditorium
3Protein metabolism: digestion and absorption, biosynthesis of amino acids and complex proteins. Functions of proteins in the human body.Lectures1.00auditorium
4Possibilities of laboratory diagnostics: classification, material and methods used, their specificity and sensitivity.Lectures1.00auditorium
5Complete blood count, blood biochemistry, blood culture, cytogenetic examination – indicators, meaning thereof, interpretation of changes.Classes1.00auditorium
6Fecal examination: fecal analysis, hidden blood, culture, biochemistry – indicators, meaning thereof, interpretation of changes.Classes1.00auditorium
7Urine analysis, the so-called microscopy, urine culture – indicators, meaning thereof, interpretation of changes.Classes1.00auditorium
8Serological test: antibody formation, antibody classes. Rapid and confirmatory tests.Classes1.00auditorium
9Laboratory diagnostics of M.tuberculosis – available methods, time of implementation and accuracy thereof. Introduction to polymer chain reaction (PCR).Classes1.00auditorium
10Histological examination – definition, indications, methods to be used, interpretation of results.Classes1.00auditorium
11Punctures: therapeutic and diagnostic indications. Type of collection. Examination possibilities. Interpretation of results.Classes1.00auditorium
12Bone marrow examination – indications, abnormalities and their interpretation.Classes1.00auditorium
Assessment
Unaided Work:
1. Preliminary theoretical preparation according to the topic of the class. 2. Independent work – a report or presentation on one of the topics of the class, which the student will have to study independently according to the specified literature sources and write a report about it or present the findings during the class.
Assessment Criteria:
1. Independent work (additional work assessment criteria – correspondence of the content of the work to the chosen topic, the student’s personal contribution, the student’s level of knowledge) – 50%. 2. Written multiple choice test – 50%.
Final Examination (Full-Time):Exam (Written)
Final Examination (Part-Time):Exam (Written)
Learning Outcomes
Knowledge:While cooperating in different fields, students will gain deeper knowledge and comprehension of biochemical processes in the human body. Students will be able to describe and classify modern laboratory diagnostic methods, their indications. Students will define how the test results are formed, what they mean, how to interpret them from a medical point of view. According to the changes in the test results, students will formulate and explain the meaning of these changes, possible causes and potential consequences.
Skills:Students will be able to independently create a data analysis plan for research aimed at human disease laboratory diagnostics, as well as to implement the developed plan; will be able to integrate laboratory examination into the design of disease diagnostic research, understanding the nature of the examination and the changes observed.
Competencies:When using patient data, students will be able to qualitatively analyse the results of disease diagnostics, differential diagnosis; will critically evaluate changes in test results not only from a statistical but also from a medical point of view. Students will be able to professionally cooperate with colleagues, using and understanding biochemical and laboratory terms. When working with patient data, students will follow the principles of medical ethics.
Bibliography
No.Reference
Required Reading
1Victor W. Rodwell, David A. Bender, Kathleen M. Botham, Peter J. Kenelly, P. Anthony Weil. Harper’s Illustrated Biochemistry, Thirty-first edition. Lange, 2018.
2Goldman L, Schafer AI. Goldman's Cecil Medicine, Twenty-Fifth Edition. Saunders, 2016
3Chernecky Cynthia C., Berger Barbara J. Laboratory tests and diagnostic procedures, Sixth edition. Elsevier, 2013.
4McPherson, Richard A., Pincus, Matthew R. Henry’s. Clinical Diagnosis and Management by Laboratory Methods, Twenty-Third edition. Elsevier, 2017.
Other Information Sources
1https://www.clinicalkey.com
2http://www.who.int/
3http://site.ebrary.com/lib/rsub
4https://jmd.amjpathol.org
5https://emedicine.medscape.com/