Analyses, services, consultations

Molecular Biology

• Isolation of nucleic acids from various tissues
• Determination of the presence, load and activation of viruses
• PCR methods

HHV-6 and HHV-7 Diagnostics

• Detection of genetically inherited virus (HHV-6A, HHV-6B, HHV-7)
• Determination of HHV-6 reactivation by analysis of viral miRNA in tissue biopsies using fluorescence in situ hybridization (FISH)

Single-cell RNA-seq (sample preparation (single and multiplexed samples), library preparation, sequencing (optional)

• Single cell RNAseq
• Single cell ATACseq
• Single cell SLAMseq
• Single core RNAseq

Total RNA-seq (sample preparation, library preparation, sequencing (optional)

• Volume SLAMseq

Southern and Northern Hybridization (radioactive methods)

• Determination and characterisation of miRNA
• Detection and characterisation of mRNA and non-coded RNA
• DNA detection

Flow cytometry / immunology

• Cell analysis - counting, phenotyping (membrane markers, internal cell markers), multiparameter analysis, cell cycle analysis
• Sorting cells by size, phenotype
• Determination of cytokines, hemokins in plasma, serum, cell supernatant (Luminex200, Varioscan, BD FACS Aria III)
• Analysis of biomarkers in oncology and immunology using flow cytometry and liquid profiling

Cell culture and analysis

• Isolation of mononuclear cells
• Maintaining cells in culture (eukaryotic cells)
• 3D organoid cultures
• Cell cultures under hypoxia conditions
• Viability / cytotoxicity of cells
• Profiling BH3 to assess drug response

Microscopy

• Confocal microscope
• Wide field fluorescence microscope

Cell isolation from patient biological samples, culturing, cryopreservation

• To generate cell lines
• For the preservation and propagation of genetic material
• For material preparation for single cell sequencing services
• For functional in vitro tests / phenotype measurements

Culturing and isolation of cell lines from patient-derived cell cultures

• Cultivate cell lines available in catalogs (ATCC, DSMZ)
• Insulating fibroblast cell lines from skin biopsy
• Generation of cancer cell cultures / lines from tumor biopsy material:
  • two-dimensional traditional adherent / suspension cell lines
  • generation of histotypical organoid cultures

Identification and characterisation of isolated cell cultures / cell lines

• Detection of mycoplasma by PCR
• Tissue of origin / tumor-specific marker determination (immunofluorescence microscopy)

Functional and biological cell assays for characterisation cell lines, compounds, biomaterials and nanomaterials

• Viability / cytotoxicity tests
• Reactive radicals formation (ROS) tests
• Cell cycle analysis

Isolation of cell cultures and generation of cell lines from patient biological samples, cryopreservation, and characterisation

Biomedical, pathology research, single-cell proteomics, microscopy, spatial transcriptomics and proteomics image processing and analysis

• AI-based image analysis for biomedical and pathological research
• Processing single cell proteomic images
• Segmentation and quantification of microscopy images
• Spatial Transcriptomics and proteomics analysis

Bioinformatics and data science

• Integration and analysis of omics data (proteomics, genomics, transcriptomics)
• Statistical modelling and machine learning for biomedical data
• FAIR data management and metadata standardization

Biometric and artificial intelligence solutions for stress detection

• AI-guided stress detection tools (multimodal biometrics: face, voice, iris)
• AI-assisted Diagnostic tools

Consulting and training in the field of bioimage processing, research methodology and statistical analysis

• Bioimage processing training (Python, OpenCV, QuPath, Fiji/ImageJ)
• Training in research methodology and statistical analysis