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Prolonged hypoxia induced differential protein signatures in BRCA1 +/- triple negative breast cancer cell lines

Project/agreement No.
Project funding
3 500.00 EUR, 100% financing from Baltic-German University Liaison Office.
This project of the Baltic-German University Liaison Office is supported by the German Academic Exchange Service (DAAD) with funds from the Foreign Office of the Federal Republic Germany.

Project realization
01.02.2019. - 31.10.2019.


Proposed project aims to analyse differential protein biosynthesis in BRCA1 +/- TNBC cell lines, cultured under prolonged hypoxia (to mimic in vivo microenvironment of breast cancer). Identified differentially expressed proteins will be analysed in respect to DSB repair signalling pathways to identify potential therapy targets for this aggressive type of breast cancer.


Breast cancer is the most common cancer in Europe (age standardized incidence 109 cases per 100 000 population, accordingly, in 2012), and results in substantial socioeconomic burden on the society. Triple-negative breast cancer (TNBC) is associated with a poor prognosis and defines a subgroup of patients who do not benefit from endocrine or anti-HER2 therapy. Although TNBC has relative chemo-sensitivity it is still characterized by aggressive clinical behaviour. In our previous studies we have proved that relapse rates and breast cancer-specific survival (BCS) outcomes for BRCA1 positive (+) TNBC is significantly better compared to BRCA1 negative (-) TNBC. This finding is in the line with proposed involvement of altered DNA double strand break (DSB) repair system in therapy response.

Project activities

  1. Protein isolation from cultured cell lines 
  2. Protein concentration determination and quality assessment with 1D gel electrophoresis 
  3. Sample preparation for mass-spectrometry 
  4. Sample analysis with LC-MSe/HDMSe 
  5. Data analysis with PLGS and bioinformatics 
  6. Identified proteins pathway analysis 
  7. Manuscript / conference thesis preparation for publication of acquired results 

Contacts: Zanda Daneberga (zanda[pnkts]danebergaatrsu[pnkts]lv)