Skip to main content

The Scientific Laboratory of Molecular Genetics conducts research in the area of the genetic causes of diseases, diagnostics of rare diseases, application of genetic biomarkers in diagnostics and disease forecasting.

The Scientific Laboratory of Molecular Genetics (SLMG) have received certificates from international external quality control schemes: Cystic Fibrosis Network, DGKL (Deutsche Vereinte Gesellschaft für Klinische Chemie und Laboratoriumsmedizin), Instand UKNEQA and EMQN (European Molecular Genetics Quality Network) and is registered with the Register of Medical Treatment Institutions under No.019218301.

Research Areas

  • Rare monogenic disorders
    • Cystic fibrosis (mucoviscidosis)
    • Wilson disease
    • Apha-1-antitrypsin deficiency
    • Kennedy disease
    • Ornithine transcarbamilase deficit
    • Crigler0Najjar syndrom etc.
  • Monogenic pathologies
    • Gilbert syndrome
    • HFE-related hereditary haemochromatosis
  • Multifactorial pathologies
    • Infertility
    • Myeloproliferative disorders (JAK2, CALR, MPL gene allelic variants)
  • Farmacogenetics
    • Azathioprine toxicity etc.

Research is conducted when permission is received from the Central Medical Ethics Committee.

Services

Technologies

  • DNA isolation from different biological materials
  • Quantitative and qualitative measurements of DNA quality
  • Sequencing service:
    • Direct sequencing
    • Fragment analysis / microsatellite analyses
  • Genotyping
    • Direct sequencing
    • PCR-RFLP, ARMS-PCR
    • qPCR, HRM, TaqMan

Pathogenic varint detection in following disorders

  • Cystic fibrosis (most frequent causative variant and whole CFTR gene sequencing)
  • Wilson's disease (most frequent causative variant and whole ATP7B gene sequencing)
  • Myeloproliferative neoplasia (JAK2  gene V617F variant, CALR gene  9th exon ind/del analysis)
  • Inherited thrombophilia (F5 gene G1691A variant, F2 (protrombine) gene G20210A variant, MTHFR gene C677T and A1298C variant analysis)
  • Male infertility (Y chromosome microdeletion analysis)

Projects

ERDF
  • ERDF project No. 2014/0048/2DP/2.1.1.1.0/14/APIA/VIAA/074. Development of a new method for the detection of genetic variations significant in reproduction. Project manager: I. Kempa. Duration: 2014-2015
  • ERDF project No KC-PI-2017/24 Test for detection of infertility genetic causes. Project manager: L. Gailīte. Duration: 2017-2020
Grants by the Latvian Council of Science
  • Latvian Council of Science Grant No. 09.1384. Expression of genetically determined modulations of liver metabolism in cases of different hepatic pathologies. Duration: 2009–2012
  • Latvian Council of Science Grant No. 09.1116. Diagnosis of human pathology caused by mitochondrial DNA mutations and analysis of etiopathogenesis. Duration: 2009–2012
  • Implementation of the sub-project “Polymorphic variants of xenobiotic metabolism genes in the Latvian population and their relation to pathology” of the Latvian Council of Science project No. 10.0010 “Genetic research of diseases etiology, pathogenesis, and human ageing processes of the Latvian population”. Duration: 2010–2013

  • Latvian Council of Science Grant No. 09.1115. “Importance of risk factors in the development of non-syndromic cleft palate, cleft lip with/without cleft palate for the Latvian population”. Duration: 2009–2011

Publications

2019
2018
2017
2016
2015
2014
  • Kreile M, Rots D, Piekuse L, Cebura E, Grutupa M, Kovalova Z, Lace B. Lack of association between polymorphisms in genes MTHFR and MDR1 with risk of childhood acute lymphoblastic leukemia. Asian Pac J Cancer Prev. 2014;15(22):9707-11.
  • Piekuse L, Kreile M, Zarina A, Steinberga Z, Sondore V, Keiss J, Lace B, Krumina A. Association between inherited monogenic liver disorders and chronic hepatitis C. World J Hepatol. 2014 Feb 27;6(2):92-7. doi: 10.4254/wjh.v6.i2.92.
  • Piekuse L., Lace B, Kreile M, Sadovska L, Kempa I, Daneberga Z, Micule I, Sondore V, Keiss J, Krumina A. Impact of the genes UGT1A1, GSTT1, GSTM1, GSTA1, GSTP1 and NAT2 on acute alcohol-toxic hepatitis. Central European Journal of biology, Vol 9 (2), February 2014, pp 125-130. doi: 10.2478/s11535-013-0249-y
  • Kevere L, Purvina S, Bauze D, Zeibarts M, Andrezina R, Piekuse L, Brekis E, Purvins I. Homocysteine and MTHFR C677T polymorphism in children and adolescents with psychotic and mood disorders. Nord J Psychiatry. 2014 Feb; 68(2):129-36. doi: 10.3109/08039488.2013.782066. Epub 2013 Apr 16. PMID: 23586533
  • Kempa I, Ambrozaitytė L, Stavusis J, Akota I, Barkane B, Krumina A, Matulevičienė A, Utkus A, Kučinskas V, Lace B. Association of BMP4 polymorphisms with non-syndromic cleft lip with or without cleft palate and isolated cleft palate in Latvian and Lithuanian populations. Stomatologija. 2014;16(3):94-101.
2013
  • Piekuse, L., Kreile, M., Zarina, A., Shteinberga, Z., Sondore, V., Keiss, J., Lace, B., Krumina, A. Association between inherited monogenic liver disorders and chronic hepatitis C. World Journal of Hepatology (In press).
  • Piekuse, L., Lace, B., Kreile, M., Sadovska, L., Kempa, I., Daneberga, Z., Micule, I., Sondore, V., Keiss, J., Krumina, A. Impact of the genes UGT1A1, GSTT1, GSTM1, GSTA1, GSTP1 and NAT2 on acute alcohol-toxic hepatitis. Central European Journal of biology, Vol 9 (2), February 2014, pp 125-130. doi: 10.2478/s11535-013-0249-y
  • Kevere, L., Purvina, S., Bauze, D., Zeibarts, M., Andrezina, R., Piekuse, L., Brekis, E., Purvins, I. Homocysteine and MTHFR C677T polymorphism in children and adolescents with psychotic and mood disorders. Nord J Psychiatry. 2014 Feb;68(2):129-36. doi: 10.3109/08039488.2013.782066. Epub 2013 Apr 16. PMID: 23586533\
  • Bauze, D., Piekuse, L., Kevere, L., Kronberga, Z., Rizevs, A., Vaivade, I., Viksne, K., Andrezina, R., Lace, B. Association a Single Nucleotide Polymorphism in Chromosome 11 with Autism Spectrum Disorder in a North-Eastern European Population. Proceedings of the Latvian Academy of Science, 2013 (In press).
  • Berza, N., Zodzika, J., Kroica, J., Reinis, A., Skadins, I., Piekuse, L., Melngaile, O., Pundure,R., Lukojanova, I., Vasina, O. Association between Lactobacillus species and bacterial vaginosis-related bacteria, and bacterial vaginosis scores in small population of pregnant Latvian women. International Journal of Collaborative Research on Internal Medicine & Public Health, 2013, Vol. 5 No. 5, pp255-264.
  • Skesters, A. P., Silova, A. A., Spadiene, A. G., Savickiene, N. Z., Rusakova, N. J., Larmane, L. T., Moisejevs, G. O. Redox status blood of patients with new-found type-2 diabetes mellitus before and after duration of administration of natural antioxidants. Prenosology and Healthy Lifestyle, 1(12), 2013, 76-79 pp.; (in Russian)
2012
  • Kevere L., Purvina S., Bauze D., Zeibarts M., Andrezina R., Rizevs A., Jelisejevs S., Piekuse L., Kreile M., Purvins I.: Elevated serum levels of homocysteinedisord as an early prognostic factor of psychiatric disorders in children and adolescents, Schizophr Res Treatment. 2012(2012:373261), pp., 2012, doi:10.1155/2012/373261. Epub, 2012, Oct 2.
  • Sadovska L., Piekuse L., Kreile M., Keiss J., Krumina A.: CCR5-32 and UGT1A1 28-mutations in HCV patients, Immunology. Vol. 137 Suppl. 1, pp. 497-497, 2012
  • Letra A., Fakhouri W., Fonseca R., Menezes R., Kempa I., Prasad J., McHenry T., Lidral A., Moreno L., Murray J., Daack-Hirsch S., Marazita M., Castilla E., Lace B., Orioli I., Granjeiro J., Schutte B., Vieira A.: Interaction between IRF6 and TGFA Genes Contribute to the Risk of Non syndromic Cleft Lip/Palate, PLoS One. 7(9), 2012, doi:10.1371/journal.pone.0045441
  • Lace B., Kempa I., Klovins J., Stavusis J., Krumina A., Akota I., Barkane B., Vieira A., Nagle E., Grinfelde I., Maulina I.: BCL3 gene role in facial morphology, Birth Defects Res. Part A-Clin. Mol. Teratol. Vol. 94(11), pp. 918-924, 2012
  • Kreile M., Daneberga Z., Ziemele I., Eglite I., Grinfelde I., Sviridova D., Krumina Z.: Shwachman diamond syndrome mimics disorder of galactose metabolisms, J. Inherit. Metab. Dis.. Vol. 35 Suppl. 1, pp. S166-S166, 2012
2011
  • Makrecka, M., Kuka, J., Liepinsh, E., Dambrova, M.: T Nikopensius, T., Kempa, I., Ambrozaityte, L., Jagomagi, T., Saag, M., Matuleviciene, A., Utkus, A., Krjutskov, K., Tammekivi, V., Piekuse, L., Akota, I., Barkane, B., Krumina, A., Klovins, J., Lace, B., Kucinskas, V., Metspalu, A.: Variation in FGF1, FOXE1, and TIMP2 genes is associated with nonsyndromic cleft lip with or without cleft palate. Birth Defects Res. Part A-Clin. Mol. Teratol. Vol. 91, pp. 218–225, 2011
  • Lace, B., Kempa, I., Piekuse, L., Grinfelde, I., Klovins, J., Pliss, L., Krumina, A., Vieira, A.R.: Association studies of candidate genes and cleft lip and palate taking into consideration geographical origin. European Journal of Oral Sciences, Vol. 119, pp. 413–417, 2011
  • Puzuka, A., Pronina, N, Grinfelde, I, Erenpreiss, J, Lejing, V, Bars, J, Pliss, L, Pelnena, I, Baumanis, V, Krumina, A.: Y chromosome-a tool in infertility studies of Latvian population. Russ. J. Genet. Vol. 47, pp. 347–353, 2011

Equipment

1111_RTU_use_science_logo_big.png

Related news