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DNA in Vitro

Study Course Description

Course Description Statuss:Approved
Course Description Version:8.00
Study Course Accepted:31.08.2021
Study Course Information
Course Code:BUMK_004LQF level:Level 7
Credit Points:2.00ECTS:3.00
Branch of Science:Biology; GeneticsTarget Audience:Dentistry
Study Course Supervisor
Course Supervisor:Agnese Zariņa
Study Course Implementer
Structural Unit:Department of Biology and Microbiology
The Head of Structural Unit:Juta Kroiča
Contacts:Riga, 16 Dzirciema Street, bmkatrsu[pnkts]lv, +371 67061584
Study Course Planning
Full-Time - Semester No.1
Lectures (count)2Lecture Length (academic hours)2Total Contact Hours of Lectures4
Classes (count)14Class Length (academic hours)2Total Contact Hours of Classes28
Total Contact Hours32
Study course description
Preliminary Knowledge:
Knowledge in biology and chemistry acquired within the framework of general secondary education and basic knowledge in molecular biology.
Objective:
To provide knowledge acquisition about DNA analysis methods used in clinical practice.
Topic Layout (Full-Time)
No.TopicType of ImplementationNumberVenue
1The secrets of DNA molecule. The structure of Human genome. Mutations. DNA polymorphism. The role of genetics in clinical dentistry.Lectures1.00auditorium
2Introduction to laboratory equipment and work in scientific laboratories. Individual experimental plan creation. Pipetting.Classes1.00auditorium
3Extraction solution preparation required for DNA. DNA extraction, extracted DNA quality assessment.Classes1.00auditorium
4Polymerase chain reaction (PCR).Classes1.00auditorium
5Horizontal and vertical electrophoresis basic principles. Analysis of PCR products in agarose gel. Restriction fragment length polymorphism analysis (RFLP).Classes1.00auditorium
6Polyacrylamide gel electrophoresis (PAAGE).Classes1.00auditorium
7Validation of the obtained results by using sequencing method as an example.Classes1.00auditorium
8DNA extraction from mouth bacteria. Sampling methods and creating sample groups. Verification of reagents.Classes1.00auditorium
9Analysis of the obtained results. Reports for the results of diagnostic genetic testing preparation.Classes1.00auditorium
10Analysis of obtained results. Significance of the results in molecular diagnosis of periodontitis.Classes1.00auditorium
11Student portfolio presentations.Classes1.00auditorium
12Genetic aspect of periodontitis.Lectures1.00laboratory
13DNA sequencing.Classes1.00laboratory
14Programmes for DNA analysis.Classes1.00laboratory
15DNA restriction and ligation.Classes1.00laboratory
16Topicalities in DNA analysis.Classes1.00laboratory
Assessment
Unaided Work:
To prepare literature review about latest achievements in molecular genetics relevant in dentistry. Individual work has 50% impact on the final evaluation.
Assessment Criteria:
Attendance of practical classes will be registered, implementation of laboratory work protocols will be controlled, evaluation of practical skills will be carried out. At the end of the course it is possible to obtain a cumulative assessment, which consists of activities in practical classes and independent work.
Final Examination (Full-Time):Exam (Written)
Final Examination (Part-Time):
Learning Outcomes
Knowledge:Upon successful completion of the course the students will be able to interpret diagnosis of genetic disease by molecular methods.
Skills:Upon successful completion of the course students will be able to extract DNA from living cells, analyse mutation by gel electrophoresis, create a plan for the screening of genetic disease.
Competencies:Students will be able to assemble theoretical knowledge and practical skills and to justify necessity of its usage in solution of clinical problems.
Bibliography
No.Reference
Required Reading
1Russell P.J. Genetics: A Molecular Approach. 3rd edition, Pearson, 2013.
2WS Klug, MR Cummings, ChA Spencer, MA Palladino, D Killian. Concepts of Genetics. Pearson, 12th ed., 2018.
Additional Reading
1Strachan T., Read A. Human Molecular Genetics. - GS, 2011
Other Information Sources
1Internets. NCBI (DNA &RNA, Genetics&Medicine, Genes &Expression, Genomes&Maps). Papildus saites tiks norādītas studiju kursa apguves laikā.