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DNA in Vitro
Study Course Description
Course Description Statuss:Approved
Course Description Version:8.00
Study Course Accepted:31.08.2021 16:38:21
| Study Course Information | |||||||||
| Course Code: | BUMK_004 | LQF level: | Level 7 | ||||||
| Credit Points: | 2.00 | ECTS: | 3.00 | ||||||
| Branch of Science: | Biology; Genetics | Target Audience: | Dentistry | ||||||
| Study Course Supervisor | |||||||||
| Course Supervisor: | Agnese Zariņa | ||||||||
| Study Course Implementer | |||||||||
| Structural Unit: | Department of Biology and Microbiology | ||||||||
| The Head of Structural Unit: | |||||||||
| Contacts: | Riga, 16 Dzirciema Street, bmk rsu[pnkts]lv, +371 67061584 | ||||||||
| Study Course Planning | |||||||||
| Full-Time - Semester No.1 | |||||||||
| Lectures (count) | 2 | Lecture Length (academic hours) | 2 | Total Contact Hours of Lectures | 4 | ||||
| Classes (count) | 14 | Class Length (academic hours) | 2 | Total Contact Hours of Classes | 28 | ||||
| Total Contact Hours | 32 | ||||||||
| Study course description | |||||||||
| Preliminary Knowledge: | Knowledge in biology and chemistry acquired within the framework of general secondary education and basic knowledge in molecular biology. | ||||||||
| Objective: | To provide knowledge acquisition about DNA analysis methods used in clinical practice. | ||||||||
| Topic Layout (Full-Time) | |||||||||
| No. | Topic | Type of Implementation | Number | Venue | |||||
| 1 | The secrets of DNA molecule. The structure of Human genome. Mutations. DNA polymorphism. The role of genetics in clinical dentistry. | Lectures | 1.00 | auditorium | |||||
| 2 | Introduction to laboratory equipment and work in scientific laboratories. Individual experimental plan creation. Pipetting. | Classes | 1.00 | auditorium | |||||
| 3 | Extraction solution preparation required for DNA. DNA extraction, extracted DNA quality assessment. | Classes | 1.00 | auditorium | |||||
| 4 | Polymerase chain reaction (PCR). | Classes | 1.00 | auditorium | |||||
| 5 | Horizontal and vertical electrophoresis basic principles. Analysis of PCR products in agarose gel. Restriction fragment length polymorphism analysis (RFLP). | Classes | 1.00 | auditorium | |||||
| 6 | Polyacrylamide gel electrophoresis (PAAGE). | Classes | 1.00 | auditorium | |||||
| 7 | Validation of the obtained results by using sequencing method as an example. | Classes | 1.00 | auditorium | |||||
| 8 | DNA extraction from mouth bacteria. Sampling methods and creating sample groups. Verification of reagents. | Classes | 1.00 | auditorium | |||||
| 9 | Analysis of the obtained results. Reports for the results of diagnostic genetic testing preparation. | Classes | 1.00 | auditorium | |||||
| 10 | Analysis of obtained results. Significance of the results in molecular diagnosis of periodontitis. | Classes | 1.00 | auditorium | |||||
| 11 | Student portfolio presentations. | Classes | 1.00 | auditorium | |||||
| 12 | Genetic aspect of periodontitis. | Lectures | 1.00 | laboratory | |||||
| 13 | DNA sequencing. | Classes | 1.00 | laboratory | |||||
| 14 | Programmes for DNA analysis. | Classes | 1.00 | laboratory | |||||
| 15 | DNA restriction and ligation. | Classes | 1.00 | laboratory | |||||
| 16 | Topicalities in DNA analysis. | Classes | 1.00 | laboratory | |||||
| Assessment | |||||||||
| Unaided Work: | To prepare literature review about latest achievements in molecular genetics relevant in dentistry. Individual work has 50% impact on the final evaluation. | ||||||||
| Assessment Criteria: | Attendance of practical classes will be registered, implementation of laboratory work protocols will be controlled, evaluation of practical skills will be carried out. At the end of the course it is possible to obtain a cumulative assessment, which consists of activities in practical classes and independent work. | ||||||||
| Final Examination (Full-Time): | Exam (Written) | ||||||||
| Final Examination (Part-Time): | |||||||||
| Learning Outcomes | |||||||||
| Knowledge: | Upon successful completion of the course the students will be able to interpret diagnosis of genetic disease by molecular methods. | ||||||||
| Skills: | Upon successful completion of the course students will be able to extract DNA from living cells, analyse mutation by gel electrophoresis, create a plan for the screening of genetic disease. | ||||||||
| Competencies: | Students will be able to assemble theoretical knowledge and practical skills and to justify necessity of its usage in solution of clinical problems. | ||||||||
| Bibliography | |||||||||
| No. | Reference | ||||||||
| Required Reading | |||||||||
| 1 | Russell P.J. Genetics: A Molecular Approach. 3rd edition, Pearson, 2013. | ||||||||
| 2 | WS Klug, MR Cummings, ChA Spencer, MA Palladino, D Killian. Concepts of Genetics. Pearson, 12th ed., 2018. | ||||||||
| Additional Reading | |||||||||
| 1 | Strachan T., Read A. Human Molecular Genetics. - GS, 2011 | ||||||||
| Other Information Sources | |||||||||
| 1 | Internets. NCBI (DNA &RNA, Genetics&Medicine, Genes &Expression, Genomes&Maps). Papildus saites tiks norādītas studiju kursa apguves laikā. | ||||||||
